📌 What is the Immunophenotyping Test?
A flow cytometry-based test used to analyze immune cell populations in bone marrow or peripheral blood.
Helps identify abnormal cells, classify blood disorders, and diagnose hematologic malignancies (leukemia, lymphoma, myeloma).
Uses Fluorescence-Activated Cell Sorting (FACS) technology on a Canto flow cytometer to detect specific cell markers.
🎯 Purpose of the Test
✅ Diagnoses and classifies leukemia, lymphoma, and multiple myeloma.
✅ Evaluates bone marrow function and blood cell composition.
✅ Helps detect minimal residual disease (MRD) post-treatment.
✅ Differentiates between reactive and malignant lymphoproliferative disorders.
✅ Assesses immune deficiencies or abnormal white blood cell populations.
📊 Normal Ranges (May vary based on lab standards and cell markers tested)
🔹 White Blood Cell (WBC) Subtypes:
Lymphocytes: 20-40%
Monocytes: 2-10%
Neutrophils: 40-60%
Eosinophils: 1-6%
Basophils: <1%
🔹 Abnormal findings may indicate leukemia, lymphoma, myelodysplastic syndromes, or immune disorders.
📝 Test Procedure
1️⃣ A blood sample or bone marrow aspirate is collected.
2️⃣ Sample is processed and labeled with fluorescent antibodies targeting specific cell markers.
3️⃣ The FACS Canto flow cytometer analyzes the sample by detecting fluorescent signals from different immune cell types.
4️⃣ A detailed immunophenotyping report helps guide diagnosis and treatment.
⚠️ Who Should Get Tested?
🔹 Patients with suspected blood cancers (leukemia, lymphoma, myeloma).
🔹 Individuals undergoing monitoring for minimal residual disease (MRD) post-treatment.
🔹 Those with abnormal blood counts or unexplained lymph node swelling.
🔹 People with suspected immune system dysfunction or deficiencies.
✅ Why is This Test Important?
✔️ Essential for early diagnosis of blood cancers.
✔️ Helps monitor treatment response & detect relapse.
✔️ Provides detailed immune profiling for personalized therapy.