What is the Anti-dsDNA Antibody - Titre Test?
The Anti-double-stranded DNA (Anti-dsDNA) Antibody - Titre test detects autoantibodies that attack double-stranded DNA. This test is mainly used to diagnose and monitor systemic lupus erythematosus (SLE), an autoimmune disease that affects multiple organs.
Normal Range:
Negative: <1:10 titer (No detectable antibodies)
Borderline/Indeterminate: 1:10 - 1:40 titer
Positive: ≥1:40 titer (May indicate autoimmune disease, particularly SLE)
(Reference values may vary depending on laboratory methods)
Purpose of the Test:
✅ Diagnose Systemic Lupus Erythematosus (SLE) – A high anti-dsDNA titer strongly suggests SLE.
✅ Monitor Disease Progression – Helps assess lupus activity and severity.
✅ Evaluate Kidney Involvement (Lupus Nephritis) – High titers may indicate lupus-related kidney damage.
✅ Differentiate Autoimmune Disorders – Helps distinguish SLE from other autoimmune conditions.
✅ Assess Symptoms – Ordered for fatigue, joint pain, skin rashes, kidney issues, and inflammation.
What Can Affect Anti-dsDNA Test Results?
🔹 High Anti-dsDNA Titers: Strongly associated with active lupus and kidney complications.
🔹 Low or Negative Anti-dsDNA: May still have SLE if ANA (antinuclear antibodies) are positive.
🔹 Temporary Increases: Infections or other autoimmune conditions can cause mild elevations.
How is the Test Performed?
A blood sample is drawn and analyzed using immunological methods, such as:
🔸 ELISA (Enzyme-Linked Immunosorbent Assay)
🔸 IFA (Indirect Immunofluorescence Assay)
🔸 Crithidia luciliae immunofluorescence test (CLIFT)
📌 When to Get Tested? If experiencing lupus symptoms or monitoring existing SLE treatment.