What is the Androstenedione (A4) Test?
The Androstenedione (A4) test measures levels of androstenedione, a steroid hormone produced by the adrenal glands, ovaries, and testes. It serves as a precursor to testosterone and estrogen and helps evaluate hormonal imbalances, adrenal disorders, and reproductive health.
Normal Range:
(Values may vary based on age, sex, and lab methods)
Men: 0.6 – 2.7 ng/mL (2.1 – 9.4 nmol/L)
Women (Pre-menopausal): 0.7 – 3.1 ng/mL (2.4 – 10.7 nmol/L)
Women (Post-menopausal): 0.3 – 1.4 ng/mL (1.0 – 4.8 nmol/L)
Children: Varies by age and development stage
Purpose of the Test:
✅ Evaluate Adrenal Gland Function – Helps diagnose adrenal hyperplasia (CAH), adrenal tumors, or adrenal insufficiency.
✅ Assess Ovarian & Testicular Disorders – Useful for diagnosing polycystic ovary syndrome (PCOS), ovarian tumors, or testicular dysfunction.
✅ Investigate Hormonal Imbalances – Helps detect excess androgen production, hirsutism (excess hair growth), and virilization.
✅ Monitor Hormone Therapy – Tracks hormone replacement or steroid use.
What Can Affect Androstenedione Test Results?
🔹 Elevated Levels: May indicate PCOS, congenital adrenal hyperplasia (CAH), Cushing’s syndrome, or adrenal/testicular tumors.
🔹 Low Levels: Can be due to adrenal insufficiency or hypogonadism.
🔹 Menstrual Cycle Variations: In women, levels fluctuate based on the menstrual cycle phase.
How is the Test Performed?
A blood sample is drawn and analyzed using immunoassays or mass spectrometry.
📌 When to Get Tested? If experiencing irregular periods, infertility, excessive hair growth, acne, deepened voice, or adrenal disorder symptoms.